How does temperature affect enzyme activity?
As temperature increases to the optimum The temperature, pH or enzyme concentration that allows the enzyme to work at its best., the kinetic energyEnergy which an object possesses by being in motion. of the enzymeProteins that act as biological catalysts, meaning they speed up reactions without being used up themselves. and substrateA substance on which enzymes act.increases, causing more collisions between the enzyme and substrate.
This causes the formation of more enzyme-substrate complexes, leading to an increase in enzyme activity.
An increase in temperature beyond the optimum causes the enzyme鈥檚 active siteRegion of an enzyme where the substrate attaches. to become denaturedWhen an enzyme鈥檚 active site loses its shape meaning it can no longer work..
This means the active site loses its important shape and can no longer form enzyme-substrate complexes, leading to a decrease in enzyme activity.
Denaturation is a permanent change.
Some enzymes (e.g. in biological washing powders) are thermostable, meaning they can work at a wide range of temperatures.
This allows biological washing powder to be used at low temperatures that saves energy and money.